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Manfred Auer

biological imaging expert: Biosciences

Media contact: Dan Krotz, 510-486-4019, dakrotz@lbl.gov

 

Manfred's primary interest lie in multiscale, multimodal imaging of macromolecular machines in their respective cellular context, as well as larger scale, cellular organization in tissues and microbial communities (biofilms). Additional areas of active scientific discovery include the molecular mechanism of hearing, breast cancer pathogenesis and progression mechanisms, organization of bacterial biofilms involved in bioremediation and lignocellulose degradation, as well as organization and degradation of plant cell walls.

Employing state-of-the art cryogenic sample preparation approaches combined with advanced 3D electron microscopic imaging techniques, Manfred Auer's group is able to image simultaneously macromolecular complexes in their native cellular, tissue or microbial community context as well as the larger scale cellular and tissue/community organization, therefore achieving a more comprehensive insight into mechanistic cellular function. These multiscale imaging capabilities are complemented by a multimodal correlative imaging approach, and altogether comprise cross-modality sample preparation and labeling techniques, correlative imaging, as well as sophisticated image analysis, including data visualization, segmentation, abstraction, model building and model representation.

Also, as Director of Physical Characterization at Joint BioEnergy Institute (JBEI), Manfred applies a variety of biophysical approaches to the study of lignocellulose-based biofuels. His research includes the mechanical stress testing of plant mutants and several advanced imaging techniques to study genetically altered feedstocks for efficient fuels synthesis.

Recent research

Manfred Auer's primary interest lie in multiscale, multimodal imaging of macromolecular machines in their respective cellular context, as well as larger scale, cellular organization in tissues and microbial communities (biofilms).

Additional, areas of active scientific discovery include the molecular mechanism of hearing, breast cancer pathogenesis and progression mechanisms, organization of bacterial biofilms involved in bioremediation and lignocellulose degradation, as well as organization and degradation of plant cell walls.

Recent Publications

  • Yin L, Verhertbruggen Y, Oikawa A, Manisseri C, Knierim B, Prak L, Jensen JK, Knox JP, Auer M, Willats WG, Scheller HV. The Cooperative Activities of CSLD2,CSLD3, and CSLD5 Are Required for Normal Arabidopsis Development. Mol Plant. 2011 Apr 6. [Epub ahead of print] PubMed PMID: 21471331.
  • Miroshnikova YA, Jorgens DM, Spirio L, Auer M, Sarang-Sieminski AL, Weaver VM. Engineering strategies to recapitulate epithelial morphogenesis within synthetic three-dimensional extracellular matrix with tunable mechanical properties. Phys Biol. 2011 Apr;8(2):026013. Epub 2011 Mar 25. PubMed PMID: 21441648.
  • Bharadwaj R, Wong A, Knierim B, Singh S, Holmes BM, Auer M, Simmons BA, Adams PD, Singh AK. High-throughput enzymatic hydrolysis of lignocellulosic biomass via in-situ regeneration. Bioresour Technol. 2011 Jan;102(2):1329-37. Epub 2010 Sep 29. PubMed PMID: 20884206.
  • Hazen TC, Dubinsky EA, DeSantis TZ, Andersen GL, Piceno YM, Singh N, Jansson JK, Probst A, Borglin SE, Fortney JL, Stringfellow WT, Bill M, Conrad ME, Tom LM, Chavarria KL, Alusi TR, Lamendella R, Joyner DC, Spier C, Baelum J, Auer M, Zemla ML, Chakraborty R, Sonnenthal EL, D'haeseleer P, Holman HY, Osman S, Lu Z, Van Nostrand JD, Deng Y, Zhou J, Mason OU. Deep-sea oil plume enriches indigenous oil-degrading bacteria. Science. 2010 Oct 8;330(6001):204-8. Epub 2010 Aug 24. PubMed PMID: 20736401.
  • Remis JP, Costerton JW, Auer M. Biofilms: structures that may facilitate cell-cell interactions. ISME J. 2010 Sep;4(9):1085-7. Epub 2010 Jul 15. PubMed PMID: 20631806.
  • Sarkar P, Bosneaga E, Auer M. Plant cell walls throughout evolution: towards a molecular understanding of their design principles. J Exp Bot. 2009;60(13):3615-35. Epub 2009 Aug 17. Review. PubMed PMID: 19687127.

Awards and Memberships

  • 2000-2002 Fellow, Jane Coffin Childs Memorial Fund for Medical Research, Agouron Fellowship in Structural Biology
  • 1999 Otto-Hahn Award, Max Planck Society
  • 1998-2000 Fellow, Human Frontier Science Program
  • 1989-1993 Fellow, Cusanuswerk
  • Member of the American Association for the Advancement of Science
  • Member of the New York Academy of Sciences
  • Member of the Harvey Society
  • Member of Biophysical Society
  • Member of Association for Research in Otolaryngology
  • Scientific Editor for Macmillan Encyclopedia of Life Science
  • Research Article Referee for Nature, Nature Structural Biology, Biophysica and Biochemica Acta, Journal of Bacteriology, Breast Cancer Research
  • Chair of Bioimaging, Life Sciences Society
  • Co-Chair: Workshop on Multiscale Biological Imaging, Data Mining and Informatics
  • Representative of Lawrence Berkeley National Laboratory to the UC Biotech Research & Education Program, Member of Executive Committee
  • Consultant for Imaging Facility of King Abdullah University for Science and Technology, Saudi Arabia
  • 1999 Research Grant Referee for the Biotechnology and Biological Sciences Research Council (BBSRC), UK

In the News

Education

Skirball Institute, New York University Medical Center & The Rockefeller University, New York, NY (dual appointment)

Ph.D. Johann-Wolfgang-Goethe-Universität, Frankfurt, Germany

EMBL, Heidelberg & MPI of Biophysics, Frankfurt, Germany

B.S., Biochemistry, University of Hannover, Germany

MRC-Laboratory of Molecular Biology, Cambridge, England

 



last updated: 2017-08-04 10:31:33